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【Objective】 Surfactants are soil conditioners to improve soil water flow and reduce soil erosion. This paper investigates their impact on water-nitrogen transport in soil, as well as yield, quality and root growth of greenhouse lettuce. 【Method】 The experiment was conducted in laboratory using a calcareous cinnamon soil. The soil was amended by three surfactants: HydravanceTM200 (T1, moisturizing and synergistic surfactant mix), Methylglucosan polyether-20 (T2, moisturizing single surfactant), and IrrigAid (T3, moisture-regulating surfactant mix), separately. For each surfactant amendment, there were two nitrogen treatments: without fertilization (N0) and nitrogen fertilization (N1). Soil without amendment by any surfactant was taken as the control (CK). During the experiment, we measured the changes in soil water content and soluble nitrogen content, as well as the growth and yield of the lettuce. 【Result】 ① Compared to CK, T1, T2 and T3 enhanced the lateral movement of water in the soil, thereby leading to an even soil water distribution and a reduction in nitrate nitrogen content in the subsoil layer (20-30 cm). ② According to the pot experiment, the above-ground fresh mass and VC content of lettuce in the T1N1 treatment increased by 26.61% and 175.61%, respectively, in addition to the 2.10 times increase in nitrogen utilization efficiency (P˂0.05). Compared to CKN1, T2N1 significantly increased VC content by 104.88%, while T3N1 significantly increased total root length and volume by 61.98% and 112.90% (P |