| Autor: |
Tsuneyuki Haga, Emi Tamechika, Jun-ichi Takahashi, Suzuyo Inoue, Michiko Seyama, Toru Miura, Yuzuru Iwasaki, Tsutomu Horiuchi |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Sensors, Vol 12, Iss 6, Pp 7095-7108 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1424-8220 |
| DOI: |
10.3390/s120607095 |
| Popis: |
We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) x 160 mm(D) x 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) x 16 mm(D) x 4 mm(H)) that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL ) passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmentaland medical testing. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
