Rapidly Functional Immobilization of Immortalized Human Hepatocytes Using Cell Adhesive GRGDS Peptide-Carrying Cellulose Microspheres
| Autor: | Naoya Kobayashi M.D., Ph.D., Takehito Taguchi, Hirofumi Noguchi, Teru Okitsu, Toshinori Totsugawa, Takamasa Watanabe, Toshihisa Matsumura, Toshiyoshi Fujiwara, Haruo Urata, Nobuyuki Kishimoto, Nobuyuki Hayashi, Shuhei Nakaji, Takuro Murakami, Noriaki Tanaka |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Cell Transplantation, Vol 10 (2001) |
| Druh dokumentu: | article |
| ISSN: | 0963-6897 1555-3892 00000000 |
| DOI: | 10.3727/000000001783986512 |
| Popis: | With the development of biotechnology, hepatic support by a hybrid artificial liver (HAL) using hepatocytes has been given much attention. Because the availability of human livers is limited, we have established a tightly regulated immortal human hepatocyte cell line, NKNT-3, for developing HAL. Because high-density cell culture allows the compactness of the HAL device and its easy use under emergency circumstances, we have developed cell adhesive GRGDS peptide-containing cellulose microspheres (GRGDS/CMS). The GRGDS/CMS efficiently immobilized NKNT-3 cells within 24 h in a stirred suspension culture. Electron microscopic examinations demonstrated glycogen granules and well-developed endoplasmic reticulum and mitochondria in NKNT-3 cells attached to the GRGDS/CMS. The cells showed ammonia clearance activity, whereas HepG2-transformed human liver cells did not remove the loaded ammonia. An efficient adenoviral delivery of the lacZ reporter gene was performed in GRGDS/CMS-immobilized NKNT-3 cells. In this study we present rapid immobilization of NKNT-3 immortal human hepatocytes using cellulose microspheres carrying GRGDS peptides. These microspheres satisfied immediate preparation of NKNT-3 cells in sufficient quantity and of adequate quality. |
| Databáze: | Directory of Open Access Journals |
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