| Autor: |
Kosuke Kazashi, Kakeru Miura, Sota Ueda, Kazuhito Utsunomiya, Mari Kiriyama, Motoki Yagisawa, Masayuki Ikeda, Masanobu Kano, Toshihide Tabata |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Neuroscience Research, Vol 207, Iss , Pp 45-48 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
0168-0102 |
| DOI: |
10.1016/j.neures.2024.05.002 |
| Popis: |
The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl− concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to the cell membrane by passive diffusion. Gramicidin deposited on the pipette orifice sometimes hampers giga-seal formation, and perforation progresses only slowly. These problems may be circumvented by delivering a high concentration of gramicidin from an intra-pipette capillary after a giga-seal is formed. We herein describe the detailed protocol of this improved method. This protocol would greatly facilitate the investigation of Cl− gradient-dependent neuronal activities. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Full Text from ScienceDirect