Serological and molecular characterization of Syrian Tomato spotted wilt virus isolates
| Autor: | Faiz ISMAEIL, Amin Amer HAJ KASSEM, Salah AL-CHAABI, Ahmed ABDULKADER, Mouhamed ALKHALAF |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Phytopathologia Mediterranea, Vol 54, Iss 1 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0031-9465 1593-2095 |
| DOI: | 10.14601/Phytopathol_Mediterr-13874 |
| Popis: | Thirty four Syrian isolates of Tomato spotted wilt virus (TSWV) collected from tomato and pepper were tested against five specific monoclonal antibodies using TAS-ELISA. The isolates were in two serogroups. Fourteen tomato and sixteen pepper isolates were similar in their reaction with MAb-2, MAb-4, MAb-5 and MAb-6, but did not react with MAb-7 (Serogroup 1). Meanwhile, four isolates collected from pepper reacted with all the MAbs used (Serogroup 2). The expected 620 bp DNA fragment was obtained by RT-PCR from six samples using a specific primer pair designed to amplify the nucleocapsid protein (NP) gene of TSWV. The PCR products were sequenced and a phylogenetic tree was constructed. Sequence analysis revealed that the Syrian TSWV isolates were very similar at the nucleotide (97.74 to 99.84% identity) and amino acid (96.17 to 99.03% identity) sequences levels. The phylogenetic tree showed high similarity of Syrian TSWV isolates with many other representative isolates from different countries. |
| Databáze: | Directory of Open Access Journals |
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