| Autor: |
Qiwei Huang, CongBao Kang |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 5, Iss 3, Pp 103278- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2024.103278 |
| Popis: |
Summary: Fragment-based drug design plays an important role in drug discovery. Protein-observed NMR experiments with isotopically labeled samples are used to probe target-ligand interactions and map the ligand-binding sites. Here, we present a protocol to perform fragment screening using NMR spectroscopy. We describe steps for producing 15N-labeled Kirsten rat sarcoma viral oncogene homolog (KRAS) G12D protein, fragment screening using 1H-15N-heteronuclear single quantum coherence (HSQC) experiment, fragment deconvolution, determining binding affinities, and mapping the fragment-binding site. This protocol provides a strategy in fragment screening. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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