Investigation of Diagnostic Biomarkers for Osteoporosis Based on Differentially Expressed Gene Profile with QCT and mDixon‐Quant Techniques
| Autor: | Shan Zhu, Aixian Tian, Lin Guo, Hua Xu, Xiaofeng Li, Zhi Wang, Feng He |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Orthopaedic Surgery, Vol 13, Iss 7, Pp 2137-2144 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1757-7861 1757-7853 |
| DOI: | 10.1111/os.13094 |
| Popis: | Objective To develop a comprehensive differential expression profile for osteoporosis based on two independent data sources. Methods Using a hindlimb unloading (HLU) rat model to mimic osteoporosis syndrome in humans (animal experiments), the significant differentially expressed mRNAs in osteoporosis were analyzed using RNA‐seq. The enriched GO terms as well as KEGG signaling pathways were also deeply investigated. Using clinical specimens to verify the functions of potential hub genes (biomarkers) for osteoporosis (clinical experiments), 128 suspected cases for osteoporosis from January 2019 to December 2020 were randomly selected and analyzed by quantitative computed tomography (QCT) as well as modified Dixon quantification (mDixon‐Quant) techniques in the Tianjin hospital. Among these, 80 patients out of 128 suspected cases were finally diagnosed as the osteoporosis group. Meanwhile, 48 patients were selected for osteopenia group. There was no significant age and gender difference across participant subgroups. The protein levels of potential hub genes (FST, CCL3, and RAPGEF4) were determined by ELISA double antibody sandwich method for osteopenia and osteoporosis groups from peripheral blood. Result In the RNA‐seq analysis, compared with control group, a total of 803 differentially expressed mRNAs were identified, including 288 up‐regulated and 515 down‐regulated mRNAs. Of these, FST, CCL3, CPE, RAPGEF4, IL6, MDFI, PDZD2, and GATM were primary hub genes (biomarkers) for osteoporosis. These differentially expressed genes were significantly enriched in GO terms related to extracellular matrix process and KEGG signaling pathways including osteoclast differentiation. In the functional experiments, the protein expression level of FST, CCL3, and RAPGEF4 displayed a specific expression pattern between osteoporosis patients and control group. The protein concentration of FST was 23.63 ± 6.39 ng/mL in osteoporosis patients compared as 48.36 ± 9.12 ng/mL in osteopenia group (P |
| Databáze: | Directory of Open Access Journals |
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