| Autor: |
Ming-Zhi Han, Ran Xu, Yang-Yang Xu, Xin Zhang, Shi-Lei Ni, Bin Huang, An-Jing Chen, Yu-Zhen Wei, Shuai Wang, Wen-Jie Li, Qing Zhang, Gang Li, Xin-Gang Li, Jian Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Journal of Experimental & Clinical Cancer Research, Vol 36, Iss 1, Pp 1-14 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1756-9966 |
| DOI: |
10.1186/s13046-017-0619-9 |
| Popis: |
Abstract Background Transgelin-2 (TAGLN2) is a member of the calponin family of actin-bundling proteins that is involved in the regulation of cell morphology, motility, and cell transformation. Here, the clinical significance and potential function of TAGLN2 in malignant gliomas were investigated. Methods Molecular and clinical data was obtained from The Cancer Genome Atlas (TCGA) database. Gene ontology and pathway analysis was used to predict potential functions of TAGLN2. RNA knockdown was performed using siRNA or lentiviral contructs in U87MG and U251 glioma cell lines. Cells were characterized in vitro or implanted in vivo to generate orthotopic xenografts in order to assess molecular status, cell proliferation/survival, and invasion by Western blotting, flow cytometry, and 3D tumor spheroid invasion assay, respectively. Results Increased TAGLN2 expression was associated with increasing tumor grade (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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