Counteracting effects on free radicals and histological alterations induced by a fraction with casearins

Autor: ÉVERTON JOSÉ FERREIRA DE ARAÚJO, GUILHERME ANTÔNIO LOPES DE OLIVEIRA, LÍVIA QUEIROZ DE SOUSA, VANDERLAN DA SILVA BOLZANI, ALBERTO JOSÉ CAVALHEIRO, ADRIANA DA ROCHA TOME, ANA PAULA PERON, ANDRÉ GONZAGA DOS SANTOS, ANTONIA MARIA DAS GRAÇAS LOPES CITÓ, CLÁUDIA PESSOA, RIVELILSON MENDES DE FREITAS, PAULO MICHEL PINHEIRO FERREIRA
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Anais da Academia Brasileira de Ciências, Vol 87, Iss 3, Pp 1791-1807 (2015)
Druh dokumentu: article
ISSN: 1678-2690
0001-3765
DOI: 10.1590/0001-3765201520150149
Popis: ABSTRACTCasearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestrisand histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 μg/mL) revealed EC50 values of 3.7, 6.4 and 0.16 µg/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 µM) and striatum (26.3, 25.4, 34.3 and 27.5 µM) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris.
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