| Autor: |
Xiaoxia Wang, Tianxiang Xu, Jiajia Jin, M. M. Ting Gao, Bing Wan, Mei Gong, Lingxiao Bai, Tangfeng Lv, Yong Song |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Pulmonary Circulation, Vol 12, Iss 2, Pp n/a-n/a (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2045-8940 |
| DOI: |
10.1002/pul2.12070 |
| Popis: |
Abstract This study aims to determine the function of topotecan (TPT) in acute lung injury (ALI) induced by sepsis. The mouse sepsis model was constructed through cecal ligation and puncture (CLP). The ALI score and lung wet/dry (W/D) weight ratio were applied to evaluate the level of lung injury. Hematoxylin–eosin staining was used to examine the role of TPT in lung tissue in a CLP‐induced ALI mouse model. Enzyme‐linked immunosorbent assay and quantitative real‐time polymerase chain reaction were used to detect the concentrations of inflammatory factors, such as interleukin‐6 (IL‐6), IL‐1β, and tumor necrosis factor‐α. Western blot was used to detect relevant protein levels in the nuclear factor‐κB (NF‐κB) pathway. Moreover, 10‐day survival was recorded by constructing the CLP model. The results indicated that TPT could improve lung tissue damage in mice and could significantly reduce lung injury scores (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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