| Popis: |
Intro: Surface and environment disinfection is an important part of infection control strategies, especially in the ongoing COVID-19 pandemic. Ozone, a highly reactive oxidant, is a widely used disinfectant in many industries including food, healthcare and water treatment. It has a broad-spectrum activity and leaves no harmful residues. However, most demonstrated efficacy has been at high ozone levels (>1ppm) which can be harmful to humans in case of exposure. Here, we undertook a study to evaluate if exposure to ozone is effective in inactivating SARS-CoV-2 and feline coronavirus (FCoV) even at low concentrations. Methods: Ozone at 0.07, 0.1 and 1.2 ppm were evaluated for its virucidal activity against SARS-CoV-2 and FCoV. An ozone gas generator (Medklinn Air + Surface Sterilizer (CerafusionTM Technology), Medklinn, Malaysia) supplied controlled levels of ozone to a custom-built chamber of 1.5 ft3 (1.5ft x 1ft x 1ft) where dry virus films containing 1 × 104 PFU of test virus were exposed to ozone gas for 0.5h, 1h, 3h, 5h, and 8h. The experiment was performed at ambient temperature (23-24oC) and relative humidity (RH) of 55% (FCoV only) and 85% (SARS-CoV-2 and FCoV). Findings: At low level of ozone of 0.1ppm, >90% reduction of both viruses was achieved after 3h exposure at 85% and 55% humidity. At 1.2ppm, >90% reduction of both viruses was achieved after 0.5h exposure at 85% humidity. Ozone at 0.07ppm, however, did not show good efficacy as reduction not exceeding 90% was achieved only after 8h exposure at 85% and 55% humidity. Conclusion: The study demonstrated that low concentration of ozone of at least 0.1 ppm reduced SARS-CoV-2 and FCoV by >90% when used at 85% humidity. The use of low level ozone presents a safer alternative for disinfecting enclosed spaces and greatly reduces any potential harmful health effects in case of accidental exposure. |
