Purification, structural characterization of an arabinogalactan from green gram (Vigna radiata) and its role in macrophage activation

Autor: Kiranmayi Ketha, Muralikrishna Gudipati
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Functional Foods, Vol 50, Iss , Pp 127-136 (2018)
Druh dokumentu: article
ISSN: 1756-4646
DOI: 10.1016/j.jff.2018.09.029
Popis: An acidic arabinogalactan (AGP-2) isolated from green gram Hemicellulose-B was purified using Sephacryl S-400 and its molecular weight was found to be ∼1200 kDa. Characterization of the AGP-2 by various structural methods indicated 1,3- β- D- Galactan backbone substituted, at O-6 with 1, 5 linked arabinofuranosyl side chains, which were further substituted either with mono/di/tri substituted arabinofuranosyl residues resulting in highly branched structure. Glucuronic acid residues are present as non reducing end terminals. AGP-2 enhanced the release of NO, TNF- α, IL-6 and IL- 1β and phagocytic capability of macrophages whereas carboxyl reduced AGP-2 failed to show the similar result indicating the importance of carboxyl groups in macrophage activation. Pretreatment of macrophages with antibodies specific to extracellular domains of TLR2, TLR4 and TREM1 significantly reduced the AGP-2 induced release of NO and TNF– α indicating the interaction of AGP-2 with TLRs to activate macrophages.
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