ASSOCIATION OF SEBORRHEIC KERATOSIS AND HUMAN PAPILLOMA VIRUS IN IMMUNE-SUPPRESSED AND IMMUNOCOMPETENT PATIENTS: A COMPARISON STUDY
| Autor: | V. A. Molochkov, L. V. Korneva, E. S. Snarskaya, E. O. Shcherbakova, A. A. Polyanskaya, O. B. Trofimova |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 34, Pp 52-55 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2072-0505 2587-9294 |
| DOI: | 10.18786/2072-0505-2014-34-52-55 |
| Popis: | Aim: To study an association between seborrheic keratosis and human papilloma virus (HPV) using quantitative analysis of viral desoxyribonucleic acid (DNA); to assess prevalence of different phenotypes of beta-HPV. Materials and methods: We examined 60 renal transplant recipients (20 of them had multiple seborrheic keratosis) and 22 immunocompetent patients with seborrheic keratosis. Control group included 49 healthy subjects. Burr biopsy samples (micro-samples) were collected in sterile conditions. After sample procession and DNA isolation using DNK-sorb-C kit (Central Research Institute for Epidemiology – CRIE), polymerase chain reaction for HPV was performed with real-time fluorescent hybridization detection. For DNA amplification and detection we used RotorGene 3000 analyzer (Corbett Research, Australia). In the beta-HPV assay, recombinant plasmids were used as positive controls and control human beta-globin gene fragments (CRIE). 4 oligo-nucleotide systems (group-specific primers and probes) were used for the detection of beta-HPV DNA. Results: Keratotic lesions of open and covered skin regions were common in renal transplant recipients. Beta-HPV DNA was more frequent in seborrheis keratomas and intact skin (81% and 55%) of renal transplant recipients compared to healthy donors (47%). Conclusion: HPV DNA was frequently detected in keratotic lesions and intact skin of renal transplant recipients. In immunocompetent patients prevalence of HPV DNA in keratotic lesions was significantly higher compared to intact skin. |
| Databáze: | Directory of Open Access Journals |
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