Molecular detection of Dirofilaria immitis in dogs and mosquitoes in Tabasco, Mexico

Autor: Oswaldo M Torres-Chable, Carlos M Baak-Baak, Nohemi Cigarroa-Toledo, Bradley J Blitvich, Ligia G Brito-Argaez, Yessenia N Alvarado-Kantun, Claudia V Zaragoza-Vera, Guadalupe Arjona-Jimenez, Lluvia G Moreno-Perez, Pablo Medina-Perez, Carlos I Machain-Williams, Julian E Garcia-Rejon
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Vector Borne Diseases, Vol 55, Iss 2, Pp 151-158 (2018)
Druh dokumentu: article
ISSN: 0972-9062
DOI: 10.4103/0972-9062.242563
Popis: Background & objectives: Dirofilaria immitis is a filarial nematode that causes heartworm disease in domestic as well as wild canines and felines; and cutaneous or pulmonary infections in humans. The purpose of the study was to estimate the prevalence of D. immitis in domestic dogs in Tabasco, Mexico and to assay mosquitoes temporally and spatially associated with dogs for evidence of infection. Methods: Blood was collected from 1050 dogs in 1039 houses during a random household survey performed in 2016 and 2017. Genomic DNA was extracted and assayed by polymerase chain reaction (PCR) using pan-filarial primers and various species-specific primers. Dog owners were interviewed using a structured questionnaire designed to collect information on factors that may impact the occurrence of filarial infection. The association between canine dirofilariasis prevalence and factors likely to impact infection was determined by univariate logistic regression analysis, followed by multivariate binomial logistic regression analysis. Indoor and outdoor resting mosquitoes were collected from houses by manual aspiration. Mosquitoes were identified according to species, homogenized and tested by PCR for filarial nematodes. Results: A total of 84 (8%) dogs were positive for D. immitis DNA, while 3 (0.3%) dogs contained Acanthocheilonema reconditum DNA. Several factors were significantly associated with D. immitis infection. For example, dogs that lived
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