| Autor: |
Patrick Kwok-Kin Lai, Judy Yuet-Wa Chan, Hin-Fai Kwok, Ling Cheng, Hua Yu, Ching-Po Lau, Ping-Chung Leung, Kwok-Pui Fung, Clara Bik-San Lau |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Journal of Traditional and Complementary Medicine, Vol 4, Iss 4, Pp 239-245 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2225-4110 |
| DOI: |
10.4103/2225-4110.139109 |
| Popis: |
The objective of the study was to identify the active fraction(s) from AR aqueous extract responsible for promoting angiogenesis using bioassay-guided fractionation. The angiogenic activity was screened by monitoring the increase of sprout number in sub-intestinal vessel (SIV) of the transgenic zebrafish embryos after they were treated with 0.06-0.25 mg/ml of AR aqueous extract or its fraction(s) for 96 h. Furthermore, the angiogenic effect was evaluated in treated zebrafish embryos by measuring the gene expression of angiogenic markers (VEGFA, KDR, and Flt-1) using real-time polymerase chain reaction (RT-PCR), and in human microvascular endothelial cell (HMEC-1) by measuring cell proliferation using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 3H-thymidine uptake assay, and cell cycle analysis. A major active fraction (P1-1-1), which was identified as glycoproteins, was found to significantly stimulate sprout formation (2.03±0.27) at 0.125 mg/ml (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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