Genetic Dissection of Phosphorous Uptake and Utilization Efficiency Traits Using GWAS in Mungbean

Autor: Venkata Ravi Prakash Reddy, Shouvik Das, Harsh Kumar Dikshit, Gyan Prakash Mishra, Muraleedhar S. Aski, Akanksha Singh, Kuldeep Tripathi, Renu Pandey, Ruchi Bansal, Madan Pal Singh, Padmavati Ganpat Gore, Manjunatha P. B., Deepali Kothari, Neha Rai, RamaKrishnan M. Nair
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Agronomy, Vol 11, Iss 7, p 1401 (2021)
Druh dokumentu: article
ISSN: 2073-4395
DOI: 10.3390/agronomy11071401
Popis: Mungbean (Vignaradiata L. Wilczek) is an early maturing legume grown predominantly in Asia for its protein-rich seeds. P deficiency can lead to several physiological disorders which ultimately result in a low grain yield in mungbean. The genetic dissection of PUpE (Puptake efficiency) and PUtE (P utilization efficiency) traits are essential for breeding mungbean varieties with a high P uptake and utilization efficiency. The study involves an association mapping panel consisting of 120 mungbean genotypes which were phenotyped for total dry weight, P concentration, total P uptake, and P utilization efficiency under low P (LP) and normal P (NP) conditions in a hydroponic system. A genotyping-by-sequencing (GBS) based genome-wide association study (GWAS) approach was employed to dissect the complexity of PUpE and PUtE traits at the genetic level in mungbean. This has identified 116 SNPs in 61 protein-coding genes and of these, 16 have been found to enhance phosphorous uptake and utilization efficiency in mungbeans. We identified six genes with a high expression (VRADI01G04370, VRADI05G20860, VRADI06G12490, VRADI08G20910, VRADI08G00070 and VRADI09G09030) in root, shoot apical meristem and leaf, indicating their role in the regulation of P uptake and utilization efficiency in mungbean. The SNPs present in three genes have also been validated using a Sanger sequencing approach.
Databáze: Directory of Open Access Journals
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