Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
| Autor: | Kristína Mravcová, Gabriela Štrkolcová, Rastislav Mucha, Eva Barbušinová, Mária Goldová, Jana Kačírová, Marián Maďar |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Annals of Agricultural and Environmental Medicine, Vol 27, Iss 3, Pp 485-488 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1232-1966 1898-2263 |
| DOI: | 10.26444/aaem/126619 |
| Popis: | Introduction and objectives The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Material and Methods The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusions This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1. |
| Databáze: | Directory of Open Access Journals |
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