Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics
| Autor: | Gabriel Hancu, Brigitta Simon, Hajnal Kelemen, Aura Rusu, Eleonora Mircia, Árpád Gyéresi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Advanced Pharmaceutical Bulletin, Vol 3, Iss 2, Pp 367-371 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2228-5881 2251-7308 |
| DOI: | 10.5681/apb.2013.059 |
| Popis: | Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all the beta-lactams, but they could be identified when supplementary information is used from color reactions and/or by using additional chromatographic systems. Conclusions: The right combination of solvent system and detection method allows the identification of the studied penicillins and cephalosporins and can be successfully used in the preliminary analysis beta-lactam antibiotics. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|