Dietary selenium supplementation, clarified egg yolk extender and slow cooling improve cryopreserved sperm characteristics of Saanen buck
| Autor: | Kambulu Lukusa, Abubeker Hassen, Khoboso C Lehloenya |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 10, Iss 1, Pp 43-48 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2305-0500 2305-0519 |
| DOI: | 10.4103/2305-0500.306437 |
| Popis: | Objective: To evaluate the effect of cooling rates, selenium supplementation, and three semen extenders on cooled and frozen-thawed Saanen buck sperm. Methods: Twenty Saanen bucks were divided into two groups: the selenium supplemented group and the control group. Ejaculates were collected once weekly by artificial vagina. The first experiment examined the effects of cooling rates and selenium supplementation on semen characteristics of Saanen bucks. Pooled semen was diluted with triladyl extender and split into two aliquots for slow and fast cooling. The second experiment explored the effect of selenium supplementation and semen extenders on post-cryopreserved sperm quality. Ejaculates from each group were divided into three aliquots and diluted with three extenders (i.e. clarified egg-yolk, whole egg yolk, Tris without egg-yolk extenders). All samples were cooled for 2 h at 4 °C and frozen at -196 °C for 24 h. The sperm characteristics such as sperm motility, acrosome integrity, normal morphology, and viability were evaluated by using a phase-contrast microscope. Results: In the first experiment, all sperm characteristics were significantly increased in selenium supplemented samples when slow cooling was used (P |
| Databáze: | Directory of Open Access Journals |
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