Insight into the Possible Association between Gene Polymorphism of IFN-γ and IL-22 with Psoriasis in Punjab, India
| Autor: | Arun Kumar Rawat, Aadil Wani, Tahseena Akhtar, Parneet Kaur, Tarun Narang, Dimple Chopra, Tej Bali Singh, Rajinder Kaur |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 12, Iss 10, Pp GC01-GC05 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2018/36381.12154 |
| Popis: | Introduction: IFN-γ and IL-22 are Th1/Th17 cytokine which have been shown to play important role in the development of autoimmune disorder like psoriasis. Aim: To identify the possible association of IFN-γ and IL-22 Single Nucleotide Polymorphisms (SNPs) with psoriasis as well as to find correlation between serum level of IL-22 and IFN-γ with severity of psoriasis. Materials and Methods: A total of 168 psoriasis patients and 152 healthy controls were included in the study. A 3 ml of blood sample was collected from each psoriasis patients and healthy control for further analysis of SNP and serum level of cytokine. A part of blood sample was used for isolation of DNA for gene polymorphism study in psoriasis patients. Gene polymorphism of IL-22 (rs1179251 and rs2227513) and IFN-γ (rs2430561, rs2069709) were studied by ARMS PCR method whereas serum level of IL-22 and IFN-γ was analysed using commercially available ELISA kits. Odds Ratio (OR) with 95% Confidence Interval (95% CI) was calculated to assess the relative risk of psoriasis for various genetic variants. Results: Serum level of IL-22 and IFN-γ was found to be (74.4±24.1 and 96.7±31.7 pg/ml respectively) significantly high (p |
| Databáze: | Directory of Open Access Journals |
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