Multicenter evaluation of two chemiluminescence and three lateral flow immunoassays for the diagnosis of COVID-19 and assessment of antibody dynamic responses to SARS-CoV-2 in Taiwan

Autor: Shey-Ying Chen, Yu-Lin Lee, Yi-Chun Lin, Nan-Yao Lee, Chia-Hung Liao, Yuan-Pin Hung, Min-Chi Lu, Jhong-Lin Wu, Wen-Pin Tseng, Chien-Hao Lin, Ming-Yi Chung, Chun-Min Kang, Ya-Fan Lee, Tai-Fen Lee, Chien-Yu Cheng, Cheng-Pin Chen, Chien-Hua Huang, Chun-Eng Liu, Shu-Hsing Cheng, Wen-Chien Ko, Po-Ren Hsueh, Shyr-Chyr Chen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Emerging Microbes and Infections, Vol 9, Iss 1, Pp 2157-2168 (2020)
Druh dokumentu: article
ISSN: 22221751
2222-1751
DOI: 10.1080/22221751.2020.1825016
Popis: ABSTRACTThis multicenter, retrospective study included 346 serum samples from 74 patients with coronavirus disease 2019 (COVID-19) and 194 serum samples from non-COVID-19 patients to evaluate the performance of five anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody tests, i.e. two chemiluminescence immunoassays (CLIAs): Roche Elecsys® Anti-SARS-CoV-2 Test (Roche Test) and Abbott SARS-CoV-2 IgG (Abbott Test), and three lateral flow immunoassays (LFIAs): Wondfo SARS-CoV-2 Antibody Test (Wondfo Test), ASK COVID-19 IgG/IgM Rapid Test (ASK Test), and Dynamiker 2019-nCoV IgG/IgM Rapid Test (Dynamiker Test). We found high diagnostic sensitivities (%, 95% confidence interval [CI]) for the Roche Test (97.4%, 93.4–99.0%), Abbott Test (94.0%, 89.1–96.8%), Wondfo Test (91.4%, 85.8–94.9%), ASK Test (97.4%, 93.4–99.0%), and Dynamiker Test (90.1%, 84.3–94.0%) after >21 days of symptom onset. Meanwhile, the diagnostic specificity was 99.0% (95% CI, 96.3–99.7%) for the Roche Test, 97.9% (95% CI, 94.8–99.2%) for the Abbott Test, and 100.0% (95% CI, 98.1–100.0%) for the three LFIAs. Cross-reactivity was observed in sera containing anti-cytomegalovirus (CMV) IgG/IgM antibodies and autoantibodies. No difference was observed in the time to seroconversion detection of the five serological tests. Specimens from patients with COVID-19 pneumonia demonstrated a shorter seroconversion time and higher chemiluminescent signal than those without pneumonia. Our data suggested that understanding the dynamic antibody response after COVID-19 infection and performance characteristics of different serological test are crucial for the appropriate interpretation of serological test result for the diagnosis and risk assessment of patient with COVID-19 infection.
Databáze: Directory of Open Access Journals
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