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Introduction: Agar dilution is the reference method recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) for the detection of fosfomycin susceptibility in Gram-negative bacteria. However, this method is difficult to apply routinely in microbiology laboratories. For this reason, commercial automated systems have been developed to detect antibiotic susceptibility more easily and practically. In our study, we aimed to evaluate the utility of commercial automated broth microdilution system in routine studies to detect fosfomycin susceptibility by comparing it with the gold standard agar dilution method. Materials and Methods: A total of 100 isolates of E. coli and K. pneumoniae isolated as urinary tract infection agents from urine samples, which were sent to Haydarpaşa Numune Training and Research Hospital, Medical Microbiology Laboratory between 01.06.2019 and 31.12.2019 were included in our study. Isolates were identified with matrix assisted lazer desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) (BioMerieux, France), and antibiotic susceptibility tests were performed with an automated broth microdilution system (Vitek-2, BioMerieux, France). Fosfomycin susceptibility was studied with the reference agar dilution method and the results were evaluated according to the EUCAST V.10.0 recommendations. In the evaluation made to compare the two methods, categorical agreement (CA), essential agreement (EA), very major error (VME), and major error (ME) values were calculated and the results were evaluated according to ISO 20776-1 criteria (CA and EA ≥90%; ME and VME |
