CD4+/CD8+ Ratio and Growth Differentiation Factor 8 Levels in Peripheral Blood of Large Canine Males Are Useful Parameters to Build an Age Prediction Model

Autor: Han-Jun Lee, Seok-Jin Hong, Seung-Soo Kim, Young-Yon Kwon, Bong-Hwan Choi, Kyung-Mi Choi, Seo-Hyeong Sheen, Myung-Jin Lee, Sun-Young Hwang, Kyoungwan Park, Younghun Joo, Hwayoung Song, Cheol-Koo Lee
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: The World Journal of Men's Health, Vol 40, Iss 2, Pp 316-329 (2022)
Druh dokumentu: article
ISSN: 2287-4208
2287-4690
DOI: 10.5534/wjmh.210003
Popis: Purpose: To build an age prediction model, we measured CD4+ and CD8+ cells, and humoral components in canine peripheral blood. Materials and Methods: Large Belgian Malinois (BGM) and German Shepherd Dog (GSD) breeds (n=27), aged from 1 to 12 years, were used for this study. Peripheral bloods were obtained by venepuncture, then plasma and peripheral blood mononuclear cells (PBMCs) were separated immediately. Six myokines, including interleukin (IL)-6, IL-8, IL-15, leukemia inhibitory factor (LIF), growth differentiation factor 8 (GDF8), and GDF11 were measured from plasma and CD4+/CD8+ T-lymphocytes ratio were measured from PBMC. These parameters were then tested with age prediction models to find the best fit model. Results: we found that the T-lymphocyte ratio (CD4+/CD8+) was significantly correlated with age (r=0.46, p=0.016). Among the six myokines, only GDF8 showed a significant correlation with age (r=0.52, p=0.005). Interestingly, these two markers showed better correlations in male dogs than females, and BGM breed than GSD. Using these two age biomarkers, we could obtain the best fit in a quadratic linear mixed model (r=0.77, p=3×10-6). Conclusions: Age prediction is a challenging task because of complication with biological age. Our quadratic linear mixed model using CD4+/CD8+ ratio and GDF8 level showed a meaningful age prediction.
Databáze: Directory of Open Access Journals
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