A T cell receptor specific for an HLA-A*03:01-restricted epitope in the endogenous retrovirus ERV-K-Env exhibits limited recognition of its cognate epitope

Autor: Erin E. Grundy, Lauren C. Shaw, Loretta Wang, Abigail V. Lee, James Castro Argueta, Daniel J. Powell, Mario Ostrowski, R. Brad Jones, C. Russell Y. Cruz, Heather Gordish-Dressman, Nicole P. Chappell, Catherine M. Bollard, Katherine B. Chiappinelli
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Mobile DNA, Vol 15, Iss 1, Pp 1-18 (2024)
Druh dokumentu: article
ISSN: 1759-8753
DOI: 10.1186/s13100-024-00333-w
Popis: Abstract Transposable elements (TEs) are often expressed at higher levels in tumor cells than normal cells, implicating these genomic regions as an untapped pool of tumor-associated antigens. In ovarian cancer (OC), protein from the TE ERV-K is frequently expressed by tumor cells. Here we determined whether the targeting of previously identified epitope in the envelope gene (env) of ERV-K resulted in target antigen specificity against cancer cells. We found that transducing healthy donor T cells with an ERV-K-Env-specific T cell receptor construct resulted in antigen specificity only when co-cultured with HLA-A*03:01 B lymphoblastoid cells. Furthermore, in vitro priming of several healthy donors with this epitope of ERV-K-Env did not result in target antigen specificity. These data suggest that the T cell receptor is a poor candidate for targeting this specific ERV-K-Env epitope and has limited potential as a T cell therapy for OC.
Databáze: Directory of Open Access Journals
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