Optimization of extraction process of Dioscorea nipponica Makino saponins and their UPLC-QTOF-MS profiling, antioxidant, antibacterial and anti- inflammatory activities
| Autor: | Yu Chang, Shuqi Wang, Jingyi Xu, Tong Zhu, Siqi Ma, Aohua Zhou, Yang Song, Mingchun Liu, Chunlian Tian |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Arabian Journal of Chemistry, Vol 16, Iss 4, Pp 104630- (2023) |
| Druh dokumentu: | article |
| ISSN: | 1878-5352 05841038 |
| DOI: | 10.1016/j.arabjc.2023.104630 |
| Popis: | Dioscorea nipponica Makino exhibits many biological activities, including relieving cough, eliminating phlegm and preventing asthma. The present study extensively evaluated the extraction process, major components, antioxidant, antibacterial and anti-inflammatory activities of total saponins extraction from Dioscorea nipponica Makino. In this study, the optimal extraction process of total saponins extract was optimized by single-factor test and response surface methodology as follows: extraction time 25 min, ethanol concentration 50 % and liquid to material ratio 55:1 ml/g, and the extraction rate was 1.72 %. Eighteen components were initially analyzed by UPLC-QTOF-MS method. Although total saponins extract exhibited mild antibacterial activities against Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus, and antioxidant activities against ferric-ion, ABTS and DPPH radicals, the perfect anti-inflammatory activity of TSE was demonstrated by significantly reducing the content of NO and the phagocytic activity in LPS induced RAW 264.7 cells, which provided a theoretical basis for the research and development of new anti-inflammatory Chinese medicine. |
| Databáze: | Directory of Open Access Journals |
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