| Autor: |
N. V. Baranova, L. A. Kirsanova, A. S. Ponomareva, E. A. Nemets, Y. B. Basok, G. N. Bubentsova, V. A. Surguchenko, V. I. Sevastianov |
| Jazyk: |
ruština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Вестник трансплантологии и искусственных органов, Vol 21, Iss 4, Pp 45-53 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1995-1191 |
| DOI: |
10.15825/1995-1191-2019-4-45-53 |
| Popis: |
Introduction. Creation of a biomedical cell product – a bioengineered pancreatic construct – is hampered by problems associated with maintaining the viability of functionally active isolated islets of Langerhans (ILs). Both biopolymer and tissue-specific scaffolds can contribute to maintaining the structure and function of isolated ILs in vitro and in vivo. The most preferred tissue-specific scaffolds for cells can be obtained via decellularized pancreas matrix scaffold (DP matrix scaffold). Objective: to conduct a comparative analysis of the secretory function of isolated ILs of rats cultured in biopolymer-based collagen-containing hydrogel (BCH) and tissue-specific DP matrix scaffold, respectively. Materials and methods. ILs from rat pancreas was isolated using classical collagenase technique with some modifications. ILs were cultured in BCH and tissue-specific scaffold under standard conditions. Tissue-specific DP matrix scaffold was obtained through decellularization of rat pancreas. The DP matrix scaffold was examined for cytotoxicity and DNA presence; it was subjected to morphological study. The secretory function of ILs was studied through enzyme-linked immunosorbent assay (ELISA). Results. The secretory function of islets cultured in BCH and DP scaffolds is significantly higher than in the monoculture of islets. The advantage of using tissue-specific DP matrix scaffolds when creating bioengineered constructs of the pancreas over BCH matrix scaffolds was identified. Conclusion. BCH and tissue-specific DP scaffolds contribute not only to preserving the viability of isolated ILs, but also to prolonging their secretory capacity for 10 days, compared with ILs monoculture. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
