Depletion of branched‐chain aminotransferase 2 (BCAT2) enzyme impairs myoblast survival and myotube formation

Autor:	Zameer N. Dhanani, Gagandeep Mann, Olasunkanmi A. J. Adegoke
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	BCAT2 branched‐chain amino acids differentiation metabolism myotube Physiology QP1-981
Zdroj:	Physiological Reports, Vol 7, Iss 23, Pp n/a-n/a (2019)
Druh dokumentu:	article
ISSN:	2051-817X
DOI:	10.14814/phy2.14299
Popis:	Abstract Much is known about the positive effects of branched‐chain amino acids (BCAA) in regulating muscle protein metabolism. Comparatively much less is known about the effects of these amino acids and their metabolites in regulating myotube formation. Using cultured myoblasts, we showed that although leucine is required for myotube formation, this requirement is easily met by α‐ketoisocaproic acid, the ketoacid of leucine. We then demonstrated increases in the expression of the first two enzymes in the catabolism of the three BCAA, branched‐chain amino transferase (BCAT2) and branched‐chain α‐ketoacid dehydrogenase (BCKD), with ~3× increase in BCKD protein expression (p
Databáze:	Directory of Open Access Journals
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