Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas
| Autor: | Keyla Vitoria Marques Xavier, Eden Silva e Souza, Erick de Aquino Santos, Michely Correia Diniz |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Acta Scientiarum: Biological Sciences, Vol 42, Pp e49877-e49877 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1679-9283 1807-863X |
| DOI: | 10.4025/actascibiolsci.v42i1.49877 |
| Popis: | Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic diversity of mcrA and 16S rRNA genes sequences available in databases. The nucleotide sequences were selected from the NCBI. The heterozygosity and molecular diversity indexes were calculated using the Arlequin 3.5 software, with plots generated by package R v3.0. The diversity and heterozygosity indices for both genes may have been influenced by the number and size of the sequences. Descriptive analysis of genetic diversity generated by sequences deposited in databases allowed a detailed study of these molecules. It is known that the organisms in a population are genetically distinct, and that, despite having similarities in their gene composition, the differences are essential for their adaptation to different environments. |
| Databáze: | Directory of Open Access Journals |
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