| Autor: |
Saima Ahmed, Andrew V. Rogers, Niamh C. Nowlan |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Royal Society Open Science, Vol 10, Iss 10 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2054-5703 |
| DOI: |
10.1098/rsos.231023 |
| Popis: |
Mechanical loading is critical for collagen network maintenance and remodelling in adult skeletal tissues, but the role of loading in collagen network formation during development is poorly understood. We test the hypothesis that mechanical loading is necessary for the onset and maturation of spatial localization and structure of collagens in prenatal cartilage and bone, using in vivo and in vitro mouse models of altered loading. The majority of collagens studied was aberrant in structure or localization, or both, when skeletal muscle was absent in vivo. Using in vitro bioreactor culture system, we demonstrate that mechanical loading directly modulates the spatial localization and structure of collagens II and X. Furthermore, we show that mechanical loading in vitro rescues aspects of the development of collagens II and X from the effects of fetal immobility. In conclusion, our findings show that mechanical loading is a critical determinant of collagen network establishment during prenatal skeletal development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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