| Autor: |
Eva Rodríguez-Traver, Eva Díaz-Guerra, César Rodríguez, Fabián Arenas, María Orera, Jaime Kulisevsky, Rosario Moratalla, Carlos Vicario |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 42, Iss , Pp - (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2019.101663 |
| Popis: |
Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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