| Autor: |
Hüseyin Erdinç Beşikcioğlu, Ümmügülsüm Yurteri, Linhan Ye, Fang Zhang, Alessandra Moretti, Ibrahim Halil Gürcinar, Alper Dogruöz, Didem Karakas, Helmut Friess, Guralp Onur Ceyhan, Rouzanna Istvanffy, Ihsan Ekin Demir |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 5, Iss 2, Pp 103132- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2024.103132 |
| Popis: |
Summary: The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibodies and mounting. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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