Meta‐analysis of genome‐wide DNA methylation and integrative omics of age in human skeletal muscle

Autor: Sarah Voisin, Macsue Jacques, Shanie Landen, Nicholas R. Harvey, Larisa M. Haupt, Lyn R. Griffiths, Sofiya Gancheva, Meriem Ouni, Markus Jähnert, Kevin J. Ashton, Vernon G. Coffey, Jamie‐Lee M. Thompson, Thomas M. Doering, Anne Gabory, Claudine Junien, Robert Caiazzo, Hélène Verkindt, Violetta Raverdy, François Pattou, Philippe Froguel, Jeffrey M. Craig, Sara Blocquiaux, Martine Thomis, Adam P. Sharples, Annette Schürmann, Michael Roden, Steve Horvath, Nir Eynon
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Cachexia, Sarcopenia and Muscle, Vol 12, Iss 4, Pp 1064-1078 (2021)
Druh dokumentu: article
ISSN: 2190-6009
2190-5991
78329582
DOI: 10.1002/jcsm.12741
Popis: Abstract Background Knowledge of age‐related DNA methylation changes in skeletal muscle is limited, yet this tissue is severely affected by ageing in humans. Methods We conducted a large‐scale epigenome‐wide association study meta‐analysis of age in human skeletal muscle from 10 studies (total n = 908 muscle methylomes from men and women aged 18–89 years old). We explored the genomic context of age‐related DNA methylation changes in chromatin states, CpG islands, and transcription factor binding sites and performed gene set enrichment analysis. We then integrated the DNA methylation data with known transcriptomic and proteomic age‐related changes in skeletal muscle. Finally, we updated our recently developed muscle epigenetic clock (https://bioconductor.org/packages/release/bioc/html/MEAT.html). Results We identified 6710 differentially methylated regions at a stringent false discovery rate
Databáze: Directory of Open Access Journals
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