| Popis: |
Deoxynivalenol (DON), a toxic secondary metabolite produced by Fusarium species that mainly infect cereals, causes huge economic loss to the agricultural industry. Biodegradation has emerged as a promising method owing to its high efficiency, specificity, and environment-friendly. The present study reports the procedures for obtaining a bacterial consortium YM-1 from the intestines of free-range chickens by anaerobic culture in Luria-Bertani (LB) and 96-well plates. The YM-1, mainly composed of Proteus_vulgaris, Tissierella_praeacuta, Fusobacterium_varium, uncultured_bacterium_g_Peptoniphilus, Bacteroides_uniformis, Bacteroides_vulgatus, Bacteroides_thetaiotaomicron, Clostridium_cochlearium, and Eubacterium_limosum, was able to effectively de-epoxidize 50 μg/mL DON by 99.2 % within 24 h under an anaerobic condition. The viability of the intestinal porcine epithelial cell line-J2 (IPEC-J2) was significantly damaged under 0.5 μg/mL, 1 μg/mL, or 2 μg/mL DON exposure. The products of DON degraded by YM-1, mainly consisting of de-epoxy-deoxynivalenol (DOM-1), were validated to be non-toxic to the IPEC-J2 cells. These results indicate the potential use of bacterial consortium YM-1 as a bio-agent for DON decontamination in foods and feeds. |
