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Objective: The goal of the present work was the identification and quantification of the phenolic profile of sweet basil using LC-ESI-MS and to evaluation the ability of this plant to synthesise manganese nanoparticles MnONPs and evaluation of their catalytic and thrombolytic activities. Material and methods: Standard procedures were used to extract bioactive molecules and test qualitative phytochemical substances. Phenolic compounds were identified and quantified using LC-ESI-MS. Furthermore, visual observation, UV–Vis and FT-IR spectroscopy, scanning and transmission electron microscopes (SEM and TEM), and XRD technique were used to verify the green synthesis of ObE-MnO NPs. Dye degradation of ObE-MnO NPs was studied using photocatalytic and sonocatalytic activities. Moreover, in vitro thrombolytic activity was evaluated by clot lysis. Results: Results of phytochemical essays showed that the aqueous extract of Ocimum basilicum L. is very rich in different chemical compounds that reduce and stabilize NPs. The results of the characterization of MnONPs show that the formation of these particles was proven by UV–Vis spectrum with the absorption peak at 405 nm, and also by FTIR spectrum at 470-522 cm−1. The study also confirmed that the MnONPs were spherical in shape with an average size of 6.52 ± 0.88 nm using TEM and SEM analysis, and that their size was 13.4 nm, tetragonal, and crystalline by XRD data. Results of catalytique activity show at a time of 240 min, an efficacy level of 70.18% and 58.90% for the photocatalytic and sonocatalytic activities. Ocimum basilicum L. extract and ObE-MnO NPs demonstrated a significant higher percentage of clot lysis by 93.33% and 94.73% respectively. Conclusion: In conclusion, the synthesized NPs made with basil extract were satisfactorily characterized utilizing a range of analytical techniques. ObE-MnO NPs also exhibit efficient catalytic and thrombolytic activities, qualifying their high degree of safety for usage in the environmental, pharmacological and medicinal domains. |
