Transport via Macropinocytic Vesicles Is Crucial for Productive Infection with Bombyx Mori Nucleopolyhedrovirus

Autor: Jinshan Huang, Chenya Li, Xudong Tang, Lin Liu, Wenbin Nan, Xingjia Shen, Bifang Hao
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Viruses, Vol 11, Iss 7, p 668 (2019)
Druh dokumentu: article
ISSN: 1999-4915
11070668
DOI: 10.3390/v11070668
Popis: Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious viral pathogen in the sericulture industry and enters host cells via macropinocytic endocytosis; however, the current understanding of the BmNPV entry mechanism remains limited. To confirm whether direct membrane fusion (DMF) results in productive BmNPV infection, DMF infectivity induced by low pH during BmNPV infection was investigated, and the infectious viral particle was traced using an eGFP-labeled virion. We found that BmNPV infection efficiently induced fluid uptake, which allowed BmNPV to bypass the cell membrane barrier via macropinocytosis. However, DMF induced by a low pH abolished the infection. While low pH is an essential condition for membrane fusion triggering, it is not sufficient for productive BmNPV infection, and DMF results in failure to transport the nucleocapsid into the nucleus. These results indicate that transport via macropinocytic vesicles facilitates BmNPV entry into the nucleus and contribute to our understanding of the BmNPV entry mechanism.
Databáze: Directory of Open Access Journals
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