| Autor: |
Marcos Rogério Sgrignoli, Danielle Alves Silva, Felipe Franco Nascimento, Danielle Antonelli Motta Sgrignoli, Gisele Alborghetti Nai, Márcia Guimarães da Silva, Michele Andrade de Barros, Maura Krähembühl Wanderley Bittencourt, Bruna Pereira de Morais, Heloíse Rangel Dinallo, Bruna Toledo Duran Foglia, Wellington Bott Cabrera, Elaine Carrion Fares, Silvia Franco Andrade |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 39, Iss , Pp - (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2019.101525 |
| Popis: |
Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNFα), before and after topical treatment with mesenchymal stem cells (MSCs). Twenty-two dogs positive for KCS were topically treated with 50 μL (1 × 106 MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNFα were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNFα), and after 6 months, there were significant (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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