| Autor: |
Rodrigo Canibano-Fraile, Emma Boertjes, Stela Bozhilova, W.W.M. Pim Pijnappel, Gerben J. Schaaf |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 2, Iss 2, Pp 100482- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100482 |
| Popis: |
Summary: Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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