Steroid and metabolic hormonal profile of porcine serum vis-à-vis ovarian follicular fluid
| Autor: | Soumen Naskar, S. Borah, Y. Vashi, R. Thomas, D. K. Sarma, J. Goswami, S. K. Dhara |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Veterinary World, Vol 9, Iss 11, Pp 1320-1323 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0972-8988 2231-0916 |
| DOI: | 10.14202/vetworld.2016.1320-1323 |
| Popis: | Aim: This study was conducted to understand whether serum level of the steroid and metabolic hormones may be indicative of their level in ovarian follicular fluid (FF) in porcine, and its influence on fertility. Materials and Methods: Ovaries from pigs (n=32) of two genetic groups, namely, native (Ghungroo; n=16) and crossbred (Hampshire × Ghungroo; n=16) were collected. Both the genetic groups comprised gilts (n=8) and sows (n=8), and sows were in luteal phase of estrus cycle. FF was aspirated from small, medium and large follicles, and centrifuged for the collection of supernatant for further analysis. Blood samples were collected from the same animals, and serum was separated. Hormones, namely, cortisol, T3, T4 and testosterone were estimated by radioimmunoassay. Two-way ANOVA was used for analysis of data considering genetic background (native or crossbred), stage of reproductive life (gilt or sow), and source of sample (serum or FF) as fixed effects. Results: It was observed that all the hormones except cortisol differed significantly (p |
| Databáze: | Directory of Open Access Journals |
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