Purified Human Bone Marrow Multipotent Mesenchymal Stem Cells Regenerate Infarcted Myocardium in Experimental Rats

Autor: Shaoheng Zhang, Zhuqing Jia, Junbo Ge, Lizhong Gong, Yanling Ma, Tao Li, Jingxuan Guo, Ping Chen, Qikuan Hu, Ping Zhang, Yonggang Liu, Zhaoping Li, Kangtao Ma, Linsong Li, Chunyan Zhou
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Zdroj: Cell Transplantation, Vol 14 (2005)
Druh dokumentu: article
ISSN: 0963-6897
1555-3892
00000000
DOI: 10.3727/000000005783982558
Popis: Recent findings suggest the feasibility of cardiac repair by transplantation of bone marrow mesenchymal stem cell (MSCs). However, it remains controversial regarding which cell type is the best source for transplanting into the ischemic heart because of lack of well-defined cell markers. In this study, we investigated the in vitro and in vivo effects of the novel multipotent marrow mesenchymal stem cells (MMSCs) from human bone marrow. Pluripotent markers (Oct4, Bmi1, and Abcg2) and vascular endothelial growth factor (VEGF) were detected by RT-PCR and immunofluorescence in MMSCs. Myocardial differentiation was induced in the expanded MMSC cultures by treatment with 5-azacyline. Expressions of VEGF in the animals transplanted with MMSCs were markedly increased in comparison with the animals injected with fibroblasts or saline at both mRNA and protein levels. VEGF expression was observed in both transplanted MMSCs and recipient cardiomyocytes by immunofluorescence. Confocal immunofluorescence microscopy revealed the specific markers for cardiomyocytes and endothelial cells in transplanted MMSCs 14 days after transplantation. Vessel count was increased and left ventricular function improved post-MMSC transplantation. These results indicate that transplantation of purified MMSCs from human bone marrow upregulated VEGF expression, enhanced angiogenesis, and improved the functional recovery following myocardial infarction in rats.
Databáze: Directory of Open Access Journals
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