Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
| Autor: | Denise Grotto, Lucas Santa Maria, Juliana Valentini, Clóvis Paniz, Gabriela Schmitt, Solange Cristina Garcia, Valdeci Juarez Pomblum, João Batista T. Rocha, Marcelo Farina |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 32, Iss 1, Pp 169-174 (2009) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 |
| DOI: | 10.1590/S0100-40422009000100032 |
| Popis: | Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment. |
| Databáze: | Directory of Open Access Journals |
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