Chemical composition and antimicrobial activity of the essential oil from the leaves and flowers of Aloysia gratissima

Autor: F.M. Santos, J.E.B.P. Pinto, S.K.V. Bertolucci, A.A. Alvarenga, M.N. Alves, M.C.T. Duarte, A. Sartoratto
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2013
Předmět:
Zdroj: Revista Brasileira de Plantas Medicinais, Vol 15, Iss 4, Pp 583-588 (2013)
Druh dokumentu: article
ISSN: 1983-084X
1516-0572
DOI: 10.1590/S1516-05722013000400015
Popis: Volatile oils from leaves and flowers of Aloysia gratissima were investigated for their chemical composition and antimicrobial activity against the bacteria Bacilus subtilis, Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa, Streptococcus pneumoniae and the Candida albicans yeast. The minimum inhibitory concentrations (MIC) of the oils were determined by the micro-dilution method, while the chemical composition was determined by GC-MS (gas chromatography mass spectrometry). The fresh leaves and inflorescence were subjected to hydrodistillation for 120 min using a Clevenger-type apparatus, and the essential oil was tested against microorganisms. High concentrations of sesquiterpenes were observed for the inflorescence, and monoterpenes were observed for the leaves. The main compounds of the inflorescence essential oil were E-caryophyllene, germacrene B, guaiol and bulnesol, while in the leaves the main compounds were trans-pinocamphone, trans-pinocarveyl acetate, and guaiol. The essential oil from the leaves showed an effect against P. aeruginosa and S. pneumonia, and the essential oil of the inflorescence showed an effect against P. aeruginosa, S. pneumonia, and Candida albicans.
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