Rapid intra-host diversification and evolution of SARS-CoV-2 in advanced HIV infection

Autor: Sung Hee Ko, Pierce Radecki, Frida Belinky, Jinal N. Bhiman, Susan Meiring, Jackie Kleynhans, Daniel Amoako, Vanessa Guerra Canedo, Margaret Lucas, Dikeledi Kekana, Neil Martinson, Limakatso Lebina, Josie Everatt, Stefano Tempia, Tatsiana Bylund, Reda Rawi, Peter D. Kwong, Nicole Wolter, Anne von Gottberg, Cheryl Cohen, Eli A. Boritz
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Druh dokumentu: article
ISSN: 2041-1723
25646508
DOI: 10.1038/s41467-024-51539-8
Popis: Abstract Previous studies have linked the evolution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic variants to persistent infections in people with immunocompromising conditions, but the processes responsible for these observations are incompletely understood. Here we use high-throughput, single-genome amplification and sequencing (HT-SGS) to sequence SARS-CoV-2 spike genes from people with HIV (PWH, n = 22) and people without HIV (PWOH, n = 25). In PWOH and PWH with CD4 T cell counts (i.e., CD4 counts) ≥ 200 cells/μL, we find that most SARS-CoV-2 genomes sampled in each person share one spike sequence. By contrast, in people with advanced HIV infection (i.e., CD4 counts
Databáze: Directory of Open Access Journals
Externí odkaz: