Comparative Evaluation of Penetration of Two Irrigating Solutions into Dentinal Tubules Using Diode Laser and Ultrasonic Activation: A Confocal Laser Microscopy Study Protocol
| Autor: | Palak Hirani, Manoj Chandak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 18, Iss 09, Pp 01-14 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2024/72799.19891 |
| Popis: | Introduction: Endodontic therapy aims to eradicate pulpal and periapical diseases by removing bacteria from root canals. Despite thorough cleaning, the smear layer obstructs canal disinfection. Root canal preparation and irrigation, particularly with sodium hypochlorite, are essential for debridement. However, conventional techniques may leave areas untreated, necessitating innovative solutions like superoxidised water and Laser-activated Irrigation (LAI) for effective treatment. Need for the Study: The effectiveness of root canal treatment is contingent upon the success of the irrigating solution and the mode used for its activation, which is directly reflected in its ability to penetrate dentinal tubules. Thus, the focus of this experimental in-vitro study is to identify the most suitable irrigating solution and an effective activation technique for better penetration into dentinal tubules. Aim: The study will evaluate and compare the penetration of Sodium Hypochlorite (NaOCl) and oxidised water irrigants into dentinal tubules using diode laser and ultrasonic activation. Materials and Methods: An experimental in-vitro study will be conducted in the Department of Conservative Dentistry and Endodontics at Sharad Pawar Dental College and Hospital, Wardha, Maharashtra, India, from August 2024 to May 2025. The approach involves categorising 60 recently extracted single-rooted teeth with fully formed apical foramina and intact occlusal morphology into six primary groups according to distinct irrigation techniques and the application of two different irrigants, resulting in 10 teeth per group. Decoronated specimens will be injected with a specific irrigant and activated using different techniques. The penetration of oxidised water into dentinal tubules by low-level laser therapy and ultrasonic activation will be evaluated and compared using confocal laser microscopy. The normality of the data will be analysed using the Kolmogorov-Smirnov test. If the data are normal, then the T-Analysis of Variance (ANOVA) test will be applied; if not, the Kruskal-Wallis test will be used at a 5% level of significance (p≤0.05). |
| Databáze: | Directory of Open Access Journals |
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