Phenotypic and genotypic characterization of multidrug resistant and extended-spectrum β-lactamase-producing Enterobacterales isolated from clinical samples in the western region in Cameroon

Autor: Omer Aurelle Nkengkana, Raspail Carrel Founou, Luria Leslie Founou, Brice Davy Dimani, Patrice Landry Koudoum, Jessica Ravalona Zemtsa, Aurelia Mbossi, Cyrielle Signe Mawout, Larissa Tchakounte Tegang, Michel Noubom
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: BMC Infectious Diseases, Vol 23, Iss 1, Pp 1-13 (2023)
Druh dokumentu: article
ISSN: 1471-2334
DOI: 10.1186/s12879-023-08742-7
Popis: Abstract Background The 2017 World Health Organization (WHO) report has listed extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) as critical pathogens for public health and requiring urgently new antibiotics. The aim of this study was to characterize phenotypically and genotypically ESBL-E isolated among clinical samples in Dschang, Cameroon. Methods A cross-sectional study was conducted during a four-month periods from February to May 2022 in the two biggest hospitals of Dschang. Clinical samples were collected and cultured on Eosin Methylene Blue agar. Suspected growing colonies were biochemically identified using the Enterosystem Kit 18R. Antimicrobial susceptibility testing (AST) was done using the Kirby Bauer disc diffusion method and interpretated according to the CA-SFM recommendations. ESBL phenotypes were double screened using CHROMagar™ ESBL and double disk synergy test (DDST). The detection of resistance genes was performed using conventional and multiplex PCR methods. Results were analyzed with SPSS (version 21) and a p-value
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