| Autor: |
Savannah L. Speckhart, Lydia K. Wooldridge, Alan D. Ealy |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 4, Iss 1, Pp 101924- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101924 |
| Popis: |
Summary: Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively.For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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