| Autor: |
Ophir Vermesh, Fariah Mahzabeen, Jelena Levi, Marilyn Tan, Israt S. Alam, Carmel T. Chan, Sanjiv S. Gambhir, James S. Harris |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
MethodsX, Vol 8, Iss , Pp 101414- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2215-0161 |
| DOI: |
10.1016/j.mex.2021.101414 |
| Popis: |
A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical components and electronics that can be hybrid packaged into a portable, handheld form factor. In conjunction, a custom fluorescence assay has been developed based on the protein-induced fluorescence enhancement (PIFE) phenomenon, enabling real-time sensor response to changes in protein concentration. Methods are described for the following: • Standard curves: Used to determine the sensitivity, dynamic range, and linearity of the VCSEL biosensor/PIFE assay system in buffer as well as in human blood and urine samples. • Comparison of VCSEL biosensor performance with a benchtop fluorimetric microplate reader. • Accuracy of the VCSEL biosensor/PIFE assay system: Evaluated by comparing sensor measurements with gold-standard clinical laboratory measurements of total protein in serum and urine samples from patients with diabetes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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