The Effect of Different Matrix Bound on the Transesterification Activity of Immobilized PPD2 Lipase

Autor:	Anita Herawati Permana, Fida Madayanti Warganegara, Deana Wahyuningrum, Akhmaloka
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	geobacillus thermoleovorans ppd2 thermostable lipase immobilization ni-nta agarose carboxmethyl chitosan transesterification. Microbiology QR1-502
Zdroj:	Journal of Pure and Applied Microbiology, Vol 12, Iss 2, Pp 513-519 (2018)
Druh dokumentu:	article
ISSN:	0973-7510 2581-690X
DOI:	10.22207/JPAM.12.2.10
Popis:	Immobilization of thermostable lipase from Geobacillus thermoleovorans PPD2 (Lip-A) were carried out on Ni-NTA agarose and carboxymethyl chitosan. Free enzyme was obtained by heterologous expression in Escherichia coli as a host cell. The enzyme showed catalytic activity for transesterification reaction. Transesterification activity of immobilized lipase on Ni-NTA agarose was increased by three fold (75.04% conversion) compared to that the free enzyme (24.65%), while the activity of immobilized lipase on carboxymethyl chitosan was slightly decreased (19.86%).
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/c44d423b6b1f4f6fa4481bbfff2eb1a8 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF