Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae
| Autor: | Pavlović R., Grujić Marica, Dojnov Biljana, Vujčić Miroslava, Nenadović Vera, Ivanović Jelisaveta, Vujčić Z. |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Archives of Biological Sciences, Vol 64, Iss 2, Pp 757-765 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0354-4664 1821-4339 |
| DOI: | 10.2298/ABS1202757P |
| Popis: | The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut. |
| Databáze: | Directory of Open Access Journals |
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