| Autor: |
Jochen Bodem, Stefanie Bock, Anita Schuch, Rebecca Moschall, Eva-Maria Schrom, Juliane Zahn, Christian Reuter, Graeme B. Jacobs, Thomas Kerkau, Axel Rethwilm, Susan Engelbrecht, Wolfgang Preiser |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Viruses, Vol 4, Iss 9, Pp 1830-1843 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1999-4915 |
| DOI: |
10.3390/v4091830 |
| Popis: |
The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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