Targeted Fluoromagnetic Nanoparticles for Imaging of Breast Cancer MCF-7 Cells
| Autor: | Javid Shahbazi, Vala Kafil, Mohammad Reza Rashidi, Hadi Valizadeh, Davoud Asgari, Jaleh Barar, Mostafa Heidari Majd, Yadollah Omidi |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Advanced Pharmaceutical Bulletin, Vol 3, Iss 1, Pp 189-195 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2228-5881 2251-7308 25791451 |
| DOI: | 10.5681/apb.2013.031 |
| Popis: | Purpose: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. Methods: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. Results: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. Conclusion: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR. |
| Databáze: | Directory of Open Access Journals |
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